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Titlebook: Epitope Mapping Protocols; Johan Rockberg,Johan Nilvebrant Book 2018Latest edition Springer Science+Business Media, LLC, part of Springer

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发表于 2025-3-21 18:07:31 | 显示全部楼层 |阅读模式
书目名称Epitope Mapping Protocols
编辑Johan Rockberg,Johan Nilvebrant
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Epitope Mapping Protocols;  Johan Rockberg,Johan Nilvebrant Book 2018Latest edition Springer Science+Business Media, LLC, part of Springer
描述This third edition volume expands on the previous editions with more detailed research on the characterization of antibody antigen interactions between different users with different requirements. The chapters in this book are divided into four parts: Part One looks at the entire native antigen and covers traditional structural biology techniques such as nuclear magnetic resonance and x-ray crystallography. Part Two talks about protein fragments derived from antigens, and discusses binding regions within antigen sequence using bacterial surface display and ELISA, for example. Part Three describes the use of surface plasmon resonance spectroscopy and biolayer interferometry, and Part Four highlights methods used to identify new antigens and assess antibody cross-reactivity. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readilyreproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Thorough and cutting-edge, .Epitope Mapping Protocols, Third Edition. is a valuable resource for anyone interested in
出版日期Book 2018Latest edition
关键词antibodies; antigens; protein-protein interactions; nuclear magnetic resonance (NMR); DNA sequencing; ELI
版次3
doihttps://doi.org/10.1007/978-1-4939-7841-0
isbn_softcover978-1-4939-9301-7
isbn_ebook978-1-4939-7841-0Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2018
The information of publication is updating

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https://doi.org/10.1007/978-1-4939-7841-0antibodies; antigens; protein-protein interactions; nuclear magnetic resonance (NMR); DNA sequencing; ELI
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Methods in Molecular Biologyhttp://image.papertrans.cn/e/image/313381.jpg
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,Animal Models of Huntington’s Disease,act with their target antigens is known as epitope mapping. The data provided by epitope mapping is extremely valuable in the process of antibody humanization, as well as in vaccine design. In many cases the epitope recognized by the antibody is a complex, discontinuous 3D conformational epitope. Ma
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Caroline Lindblad,Eric Peter Thelinine design. Solution NMR spectroscopy is particularly well suited to the residue level characterization of intermolecular interfaces, including antibody-antigen interactions, and thus to epitope identification. Here, we describe the use of NMR for residue level characterization of protein epitopes,
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Immunity in genetically obese rodents,tions in a natural biological context. As the analyte interacts with the receptors still intact on the cell surface, the experimental data provides complete dynamics and complexity of the interaction, thereby generating highly informative data. Attana’s cell-based biosensor platform can be used to o
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