Titlebook: Epithelial Cell Culture; Methods and Protocol Mario Baratta Book 2024Latest edition The Editor(s) (if applicable) and The Author(s), under

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Isolation, Culture, and Microscopic Imaging of Guinea Pig Primary Gastric Tissue Cells, methods of microscopic imaging of cells are shown, including monitoring the ability of cells to migrate as a determinant of their activity. The primary cells that expanded in cell cultures in vitro have characteristics of natural cells and facilitate studying both the spontaneous and induced biolog

NIP

Method for Two-Dimensional Epithelial Monolayer Formation Derived from Mouse Three-Dimensional Smal(ISCs), which support the high regenerative capacity of the intestinal epithelium. With the establishment of the three-dimensional (3D) organoid culture method, the cellular and molecular mechanisms of differentiation, proliferation, and maintenance of ISCs have been widely analyzed. However, the sp

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Human Hepatic Spheroid Coculture Model for the Assessment of Drug-Induced Liver Injury,regulatory agencies. Preclinical testing has served as a foundation for the evaluation of the potential risks and effectiveness of investigational new drug (IND) products in humans. However, current two-dimensional (2D) in vitro human primary hepatocyte (HPH) culture systems cannot accurately depict

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In Vitro Porcine (Explant) Colon Culture,amples are not always suitable for different analytical techniques or to answer the research question. In vitro cell culture models are generally focused on recreating a specific characteristic of an organ and are limited to a single cell population that does not display the characteristic tissue ar

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Bioencapsulation of Oocytes and Granulosa Cells,m alginate forms strong gels when jellified with barium ions, allowing the self-organization of cells into a 3D structure. This method of encapsulation is simple and cheap, allowing the culture of cells in a three-dimensional fashion.

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Culturing and Differentiation of Patient-Derived Ectocervical Epithelial Stem Cells Using Air-Liquipregnancy. To understand the complex cellular and molecular mechanisms of cervical biology and disease, reliable in vitro models are vital. We present an efficient method to isolate and cultivate epithelial stem cells from ectocervical tissue biopsies. This method combines enzymatic digestion, mecha

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Ovine Trophoblast Cells: Cell Isolation and Culturing from the Placenta at the Early Stage of Pregns trophoblast cells. Normal trophoblast functionality plays a key role during the whole pregnancy, especially in the first stage of placentation. This chapter explains the techniques to obtain sheep primary trophoblast cells from the early placenta. Overall, procedures for cell isolation, culture, c

CAMEO

Amniotic Membrane and Amniotic Epithelial Cell Culture,o its resistant extracellular matrix and to the large number of bioactive molecules released by its cells. To this regard, ovine amniotic epithelial cells (AECs) are a subset of placental stem cells with great regenerative and immunomodulatory properties. Indeed, either oAEC or AM have been object o

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Evaluation of the Epithelial Barrier Integrity in Primary Cultures of Pig Mammary Epithelial Cells,omise homeostasis. The ability to characterize biological barriers in vitro is an important study tool especially used for the intestinal barrier, the blood-brain barrier, and the lung barrier. The strength and integrity of biological barriers may be assessed by the measurement of the transepithelia

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