Titlebook: Epithelial Cell Culture; Methods and Protocol Mario Baratta Book 2024Latest edition The Editor(s) (if applicable) and The Author(s), under

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Human Minor Salivary Glands: A Readily Available Source of Salivary Stem/Progenitor Cells for Regennds needed to restore saliva production in patients with chronic xerostomia, or dry mouth. Methods were developed previously to isolate human stem/progenitor cells (hS/PCs) from major salivary glands (parotid/submandibular). Abundant minor salivary glands located in readily accessible locations in t

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Salivary Organotypic Tissue Culture: An Ex-vivo 3D Model for Studying Radiation-Induced Injury of H Thus, this 3D model biologically mimics in vivo conditions better than commonly used 2D culture in vitro models. Here, we provide a detailed workflow for generating live 3D organotypic tissue slices from patient-derived freshly resected salivary glandular tissues. We also cover the processing of th

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