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Titlebook: Epithelial Cell Culture; Methods and Protocol Mario Baratta Book 2018 Springer Science+Business Media, LLC, part of Springer Nature 2018 ca

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Isolation of Human Gastric Epithelial Cells from Gastric Surgical Tissue and Gastric Biopsies for Prtments of the gastric pit-gland units mediate these functions. We successfully developed a method for the isolation and growth of normal human gastric epithelial cells using biopsies or surgically resected tissues as the source of the cells. Gastric epithelial cell aggregates were released from the
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Establishment of Human- and Mouse-Derived Gastric Primary Epithelial Cell Monolayers from Organoidsse. Here we present a comprehensive protocol for the establishment and culture of human- and mouse-derived 3-dimensional gastric organoids transferred to 2-dimensional gastric epithelial cell monolayers. We introduce two methods that include the establishment of monolayers from: (1) intact organoids
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Murine and Human Mammary Cancer Cell Lines: Functional Tests,ted from the tumor, cells are subjected to multiple tests that dissect their ability to growth, migrate, degrade the surrounding stroma, produce 3-dimensional structures and differentiate. Targeted inhibitors, when added to these tests, are used to unravel how specific growth factors, receptors, and
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Primary Cultures of Olfactory Neurons from the Avian Olfactory Epithelium,ut the adult lifespan. Structurally and functionally mature olfactory neurons are generated in vitro from non-neuronal precursors in a process that resembles the in vivo counterparts. This chapter describes a technique for culture of olfactory neurons from the avian olfactory epithelium of embryonic
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