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Titlebook: Epithelial Cell Culture; Methods and Protocol Mario Baratta Book 2018 Springer Science+Business Media, LLC, part of Springer Nature 2018 ca

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书目名称Epithelial Cell Culture
副标题Methods and Protocol
编辑Mario Baratta
视频video
概述Includes cutting-edge methods and protocols for the study of epithelial cell culture.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from t
丛书名称Methods in Molecular Biology
图书封面Titlebook: Epithelial Cell Culture; Methods and Protocol Mario Baratta Book 2018 Springer Science+Business Media, LLC, part of Springer Nature 2018 ca
描述.This detailed book explores the most current techniques to study systems and epithelial cell culture. Beginning with an overview, the volume then continues to detail systems that seek to mimic the three-dimensional organization, epithelial cells from different organs, gastrointestinal system, thyroid, salivary gland, ovary, mammary gland, and olfactory epithelial tissue. Cell culture is a fundamental technique in both medical research and drug discovery and  two-dimensional (2D) culture has been the preferred method, due to the ease with which cell monolayers can be induced to proliferate on planar surfaces. The book propose several functional assay useful to test  cell activities. Further,  The past decades have witnessed significant efforts toward the development of three-dimensional (3D) cell cultures. Today, 3D cell cultures are emerging not only as a new tool in early drug discovery, but also as potential therapeutics to treat disease. Written forthe highly successful .Methods in Molecular Biology. series, chapters include the kind of detail and key implementation advice that leads to excellent results in the lab..
出版日期Book 2018
关键词cancer mammary cell lines; transcriptome; Pig blastocyst culture; Pancreatic bio-Scaffold; cancer stem c
版次1
doihttps://doi.org/10.1007/978-1-4939-8600-2
isbn_softcover978-1-4939-9339-0
isbn_ebook978-1-4939-8600-2Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2018
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Differentiation of Gastrointestinal Cell Lines by Culture in Semi-wet Interface,n from the effects of other bodily functions, such as hormonal and immune responses. However, cells that do not form a tight epithelium, polarize or secrete mucins lack some of the important protection mechanisms intrinsic to epithelial cells in vivo, increasing their susceptibility to external agen
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Three-Dimensional Cell Culture Model Utilization in Renal Carcinoma Cancer Stem Cell Research,s. The choice of proper culture media enables cancer stem cells proliferation in primary and stable cell lines. To obtain renal cell cancer stem-like phenotype, we employed media dedicated for mesenchymal cells and adult stem cells. Developed RCC cell line 3D culture system enables effective drug te
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Bovine Granulosa Cell Culture,l species investigated, cattle have become an excellent model for in vitro studies on follicular biology, both because of their resemblance with humans in terms of follicular biology and the importance of reproductive failure as a cause of lost productivity in the dairy industry. In this chapter, we
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3D Model Replicating the Intestinal Function to Evaluate Drug Permeability,other models are used in the beginning of any scientific discovery, the in vitro models. The relevance of in vitro cell based culture models for studying intestinal drug absorption and transcytosis during early stages of drug development is undeniable. Several in vitro co-culture models have been de
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