Titlebook: Epigenomics; Methods and Protocol Izuho Hatada,Takuro Horii Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive lice

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Solubilization of Mouse Sperm Chromatin for Sequencing Analyses Using a Chaperon Proteins us unable to solubilize the chromatin due to its resistance to sonication and enzymes usually used for chromatin fragmentation in somatic cells. Even when intense enzymatic treatment is applied, it appears to solubilize only certain portions of sperm chromatin presumably because of the heterogeneo

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Regulation of Gene Expression Using dCas9-SunTag PlatformsA methylation and histone modifications, and epigenetic changes can play a key role in modifying gene expression. With the advent of genome editing technologies, it has become possible to manipulate the epigenome of specific genomic regions to control gene expression. In particular, CRISPR–Cas9 syst

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Book 2023s on troubleshooting and avoiding known pitfalls. .Authoritative and practical, .Epigenomics: Methods and Protocols. serves as an ideal resource for researchers looking to further expand the utility and scope of epigenomics research..

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1064-3745 and tips on troubleshooting and avoiding known pitfalls. .Authoritative and practical, .Epigenomics: Methods and Protocols. serves as an ideal resource for researchers looking to further expand the utility and scope of epigenomics research..978-1-0716-2726-6978-1-0716-2724-2Series ISSN 1064-3745 Series E-ISSN 1940-6029

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https://doi.org/10.1007/978-1-4615-6424-9s, regardless of their DNA methylation status at the CG site—and enables the measurement of DNA methylation levels at 5% ~ 10% of all CpG sites in the mammalian genome. RRBS has been utilized in a large number of studies as a cost-effective method to investigate DNA methylation patterns, mainly at g