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Titlebook: Epigenomics; Methods and Protocol Izuho Hatada,Takuro Horii Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive lice

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发表于 2025-3-21 17:01:20 | 显示全部楼层 |阅读模式
书目名称Epigenomics
副标题Methods and Protocol
编辑Izuho Hatada,Takuro Horii
视频video
概述Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Epigenomics; Methods and Protocol Izuho Hatada,Takuro Horii Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive lice
描述This detailed volume provides a comprehensive collection of protocols for epigenomic research, powering our ability to analyze epigenetic modifications across the entire genome. Beginning with methods used to investigate epigenomic modifications such as DNA methylation, histone modifications, and chromatin structure, the book continues with methods for manipulating the epigenome, including platforms for epigenome editing, inducible systems for epigenome editing, and epigenetically modified animals. Written for the highly successful .Methods in Molecular Biology. series, chapters feature introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and practical, .Epigenomics: Methods and Protocols. serves as an ideal resource for researchers looking to further expand the utility and scope of epigenomics research..
出版日期Book 2023
关键词Epigenetic modifications; CRISPR; dCas9; Next Generation Sequencing (NGS); Epigenome editing
版次1
doihttps://doi.org/10.1007/978-1-0716-2724-2
isbn_softcover978-1-0716-2726-6
isbn_ebook978-1-0716-2724-2Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
The information of publication is updating

书目名称Epigenomics影响因子(影响力)




书目名称Epigenomics影响因子(影响力)学科排名




书目名称Epigenomics网络公开度




书目名称Epigenomics网络公开度学科排名




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书目名称Epigenomics被引频次学科排名




书目名称Epigenomics年度引用




书目名称Epigenomics年度引用学科排名




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书目名称Epigenomics读者反馈学科排名




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发表于 2025-3-21 20:30:22 | 显示全部楼层
https://doi.org/10.1007/3-540-11571-4igenome states enables a detailed analysis of the impact of epigenome status on transcriptional dynamics. In this chapter, we describe a method for quantifying distances between . gene and clusters of cofactor BRD4 using the STREAMING-tag system in mouse embryonic stem cells.
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https://doi.org/10.1007/978-3-319-08897-6ust activation of target genes even when strongly silenced. In this chapter, we describe our protocol to design, construct, and validate the TREE-mediated target gene activation in cultured human cells.
发表于 2025-3-22 06:04:23 | 显示全部楼层
Book 2023s across the entire genome. Beginning with methods used to investigate epigenomic modifications such as DNA methylation, histone modifications, and chromatin structure, the book continues with methods for manipulating the epigenome, including platforms for epigenome editing, inducible systems for ep
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https://doi.org/10.1007/978-1-4615-6424-9 Our protocols typically require nine cycles of polymerase chain reaction (PCR) amplification to obtain a sufficient amount of library for sequencing when 100 ng of genomic DNA is used as a starting material; moreover, it takes 3 days to complete library preparation and quality control procedures for up to eight samples.
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An Authentic Account of Adam Smithilized embryos, followed by sonication. The nucleoplasmin treatment dramatically increased the efficiency of sperm chromatin solubilization, while a relatively large amount of recombinant nucleoplasmin was required. Here, we describe an improvement of nucleoplasmin method with a less amount of recombinant protein and a shorter reaction time.
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