Titlebook: Epidermal Cells; Methods and Protocol Kursad Turksen Book 2010Latest edition Humana Press 2010 DNA.Endoplasmatisches Reticulum.Fische.Micro

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https://doi.org/10.1007/978-1-60761-380-0DNA; Endoplasmatisches Reticulum; Fische; Microarray; Precursors; Progenitors; Proteomics; Vivo; gene expres

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https://doi.org/10.1007/978-3-642-47558-0se primitive keratinocytes give rise to the progenitors, which are the proliferating keratinocytes and which can be defined as early to late progenitors, according to their differentiation status. Because of the intrinsic heterogeneity of the basal layer, the development of new methods suitable for

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Allgemeine und Spezielle ElektrochirurgieIn this perspective, it is important to define minimal culture conditions which allow proper growth and differentiation of epithelial cells in vitro. We propose a simple experimental system which permits the generation of three-dimensional epidermal constructs using a collagen layer as a scaffold mi

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Martin Kirschner,N. Guleke,O. Kleinschmidtne of defence against environmental physical, chemical and biological agents. The subsequent layer, the dermis, which is mainly formed by fibroblasts, has a more supportive function, containing large amounts of collagen, blood vessels and nerve endings and is less directly affected by external insul

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https://doi.org/10.1007/978-3-642-91205-4s. In this case, recovery and cultivation of keratinocytes from these animals provide an opportunity for in vitro studies. Working with isolated keratinocytes is also interesting for certain experiments which cannot be performed in live animals..Primary human keratinocytes can be kept in culture for

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https://doi.org/10.1007/978-3-642-91566-6tions. These conditions have proven adequate for studies of epidermal differentiation by measurements of the expression of typical early and late differentiation markers. The chapter also deals with the use of quantitative RT-PCR in order to determine the epidermal marker gene expression levels by c

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