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Titlebook: Epidermal Cells; Methods and Protocol Kursad Turksen Book 2010Latest edition Humana Press 2010 DNA.Endoplasmatisches Reticulum.Fische.Micro

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发表于 2025-3-21 19:34:30 | 显示全部楼层 |阅读模式
书目名称Epidermal Cells
副标题Methods and Protocol
编辑Kursad Turksen
视频video
概述Covers numerous state-of-the-art approaches to analyze epidermal differentiation.Provides step by step reproducible protocols for novices and experienced investigators.Comprises a comprehensive collec
丛书名称Methods in Molecular Biology
图书封面Titlebook: Epidermal Cells; Methods and Protocol Kursad Turksen Book 2010Latest edition Humana Press 2010 DNA.Endoplasmatisches Reticulum.Fische.Micro
描述.In recent years, our ability to understand and manipulate epidermal cells has increased tremendously, opening significant new possibilities in both basic science research and in regenerative medicine, including would healing and transplantation. .Epidermal Cells: Methods and Protocols, Second Edition. expands upon the popular first edition by bringing together a panel of experienced basic and clinical researchers to describe in step-by-step detail the powerful methods they have developed and optimized to analyze and manipulate epidermal cell precursors and mature epidermal cells. These protocols cover different methods and models for culturing epidermal cells, for enriching very early epidermal progenitors, as well as for studying epidermal cell commitment and differentiation both in vitro and in vivo. Topics of special interest include the derivation, characterization, and utility of epidermal stem cells, mature epidermal cells and their characterization, and applications inregenerative medicine. The protocols follow the successful .Methods in Molecular Biology.™ series format, providing introductions to their respective topics, lists of the necessary materials and reagents, and
出版日期Book 2010Latest edition
关键词DNA; Endoplasmatisches Reticulum; Fische; Microarray; Precursors; Progenitors; Proteomics; Vivo; gene expres
版次2
doihttps://doi.org/10.1007/978-1-60761-380-0
isbn_softcover978-1-61779-663-0
isbn_ebook978-1-60761-380-0Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 2010
The information of publication is updating

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发表于 2025-3-22 00:09:37 | 显示全部楼层
Functional Investigations of Keratinocyte Stem Cells and Progenitors at a Single-Cell Level Using Mse primitive keratinocytes give rise to the progenitors, which are the proliferating keratinocytes and which can be defined as early to late progenitors, according to their differentiation status. Because of the intrinsic heterogeneity of the basal layer, the development of new methods suitable for
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Expression and Analysis of Exogenous Proteins in Epidermal Cells,f their differentiation status allows the biochemical and molecular characterization of multiple proteins. We review methods to analyze exogenous protein abundance in transfected keratinocytes by immunoblot and immunoprecipitation. We also present protocols to determine the subcellular distribution
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