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Titlebook: Enzyme Cascade Design and Modelling; Selin Kara,Florian Rudroff Book 2021 Springer Nature Switzerland AG 2021 metabolic engineering.indust

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Perspectives, nature’s catalysts can be boosted by combining them either with other enzymes, i.e. multi-enzymatic cascades or with chemo-catalysts, i.e. chemo-enzymatic cascades. The early developments in the field of enzymatic cascade reactions have led to a new phase in which multi-catalytic systems can now be
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Introduction,mes becomes significant when they are coupled with other enzymes or with chemo-catalysts to synthesize value-added products at industrially relevant product titers. Joining the forces from different disciplines for the use of enzymatic cascade reactions will pave the way for the use of nature’s catalysts to produce products of our need.
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Multi-Enzymatic Cascades In Vivo,ns in organic synthesis offers several advantages over the classical step-by-step approach. In this chapter, pros and cons of enzymatic cascades in living cells are described and the current status of the design and the application of them are highlighted.
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Enzyme Cascade Process Design and Modelling,t synthesis, is a particularly attractive route for scale-up. In this chapter, the concept of cascade process design using modularization will be further outlined and the specific role of mathematical modelling in both enzyme stoichiometric balancing, as well as optimization of individual cascade modules and the complete process will be discussed.
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Enzyme Cascade Reaction Engineering,he implementation of an enzymatic cascade are discussed and the introduced equations are exemplarily illustrated for a cascade reaction. In order to highlight different emerging approaches for cascade engineering, miniaturized flow bioreactors are discussed, and examples of recent publications are presented.
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