Titlebook: Enhancer RNAs; Methods and Protocol Ulf Andersson Ørom Book 2017 Springer Science+Business Media New York 2017 long ncRNAs.transcription.ge

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Book 2017ncRNAs) expressed from enhancers. Chapter detail both wet-lab and dry-lab techniques and annotating long ncRNAs and exploring transcription by assessing where transcription starts and generally how it occurs.Written in the highly successful .Methods in Molecular Biology .series format, chapters incl

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Deciphering Noncoding RNA and Chromatin Interactions: Multiplex Chromatin Interaction Analysis by Pof chromatin. Chromatin Interaction Analysis by Paired-End Tag Sequencing (ChIA-PET) is a valuable and powerful technique in molecular biology which allows the study of unbiased, genome-wide de novo chromatin interactions with paired-end tags. Here, we describe the standard version of ChIA-PET and a Multiplex ChIA-PET version.

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https://doi.org/10.1007/978-1-349-12224-0lification (RCA). This assay detects native RNA in a sequence specific and single RNA sensitive manner, and PLA allows for the quantification and localization of protein–mRNA interactions with single-interaction sensitivity.

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Gentry

https://doi.org/10.1007/978-94-017-4740-0is fractionation protocol, which can be readily applied to many mammalian cell types. For the study of long noncoding RNAs and enhancer RNAs in regulation of transcription especially the preparation of chromatin-associated RNA can contribute significantly to further developments.

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The philosophy of Jules Lachelieranscript at the site of transcriptional elongation, thereby permitting analysis of dynamics between the two transcript species in single cells. Our approach is not limited to eRNAs, but can be implemented on other transcripts.

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https://doi.org/10.1007/978-1-349-19731-6dentify RNA–chromatin associations on a large scale. CHART-based strategies can be applied to determine the nature and extent of long noncoding RNA (long ncRNA) association with chromatin genome-wide and identify direct long ncRNA transcriptional targets.

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