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Titlebook: Enhancer RNAs; Methods and Protocol Ulf Andersson Ørom Book 2017 Springer Science+Business Media New York 2017 long ncRNAs.transcription.ge

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or distally located target genes. Enhancers have many features that have been discovered using genomic analyses. Recent studies have shown that active enhancers recruit RNA polymerase II (Pol II) and are transcribed, producing enhancer RNAs (eRNAs). GRO-seq, a method for identifying the location and
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https://doi.org/10.1007/978-1-349-12224-0through their interactions with specific cis-acting elements within target RNAs. Here, we describe a novel method to detect protein–mRNA interactions, which combines FLAG-peptide modified, multiply-labeled tetravalent RNA imaging probes (FMTRIPs) with proximity ligation (PLA), and rolling circle amp
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https://doi.org/10.1007/978-1-349-15243-8y used for functional genome studies and is partially replacing classical homologous recombination methods in different aspects. CRISPR/Cas9-derived tools indeed allow the production of a wide-range of engineered mutations: from point mutations to large chromosomal rearrangements such as deletions,
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https://doi.org/10.1007/978-1-4939-4035-6long ncRNAs; transcription; genome-wide; RNA manipulation; siRNA; antisense oligos
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