Titlebook: Enhancer RNAs; Methods and Protocol Ulf Andersson Ørom Book 2017 Springer Science+Business Media New York 2017 long ncRNAs.transcription.ge

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Visualization of Enhancer-Derived Noncoding RNA,anscript at the site of transcriptional elongation, thereby permitting analysis of dynamics between the two transcript species in single cells. Our approach is not limited to eRNAs, but can be implemented on other transcripts.

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Mapping Long Noncoding RNA Chromatin Occupancy Using Capture Hybridization Analysis of RNA Targets dentify RNA–chromatin associations on a large scale. CHART-based strategies can be applied to determine the nature and extent of long noncoding RNA (long ncRNA) association with chromatin genome-wide and identify direct long ncRNA transcriptional targets.

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,Detecting Long-Range Enhancer–Promoter Interactions by Quantitative Chromosome Conformation Capturech less frequent. 3C is becoming increasingly widespread in its use for understanding genome organization. Here we provide a protocol for quantitative 3C using real-time PCR analysis, along with essential quality controls and normalization methods.

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Targeted Gene Activation Using RNA-Guided Nucleases,bes key experimental design considerations, methods for plasmid construction, gene delivery protocols, and procedures for analysis of targeted gene activation in mammalian cell lines using CRISPR-Cas transcription factors.

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