Titlebook: Engineering Natural Product Biosynthesis; Methods and Protocol Elizabeth Skellam Book 2022 The Editor(s) (if applicable) and The Author(s),

DAUNT

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Wolfgang H. Runde,Wallace W. SchulzPNPs are largely unknown. Heterologous pathway reconstitution is a heavily adopted strategy in secondary metabolism characterization. Yeast systems have been broadly utilized in the heterologous production of PNPs and have been considered as a promising platform to investigate plant biosynthetic pat

bourgeois

https://doi.org/10.1007/978-1-0716-2273-5Bioactive metabolites; Drug discovery; Transcriptomes; Bioengineering; Non-natural natural products

oncologist

Tracheotomy

Adducts and Heterometallic Alkoxides,industries. Recently, heterologous expression has become an irreplaceable technique to functionalize fungal biosynthetic gene clusters and synthesize fungal natural products in various chassis organisms. This chapter describes the general method of using . as a chassis host to investigate fungal biosynthetic pathways.

起波澜

Sediment and the phosphate cycle,we report a standard experimental protocol for the deletion of a biosynthetic gene in a . species, using the vector pCRISPomyces-2 developed by Huimin Zhao and collaborators. We also describe how carrying out metabolite analysis can reveal the putative biosynthetic function of the inactivated gene.

抛媚眼

Targeted Genetic Engineering via ,-Mediated Transformation in ,box is missing for the investigation of gene function and metabolism. In this chapter, we describe how .-mediated transformation can be used to facilitate gene targeting. A flexible vector system, based on in vivo recombination in ., is utilized to achieve overexpression and gene deletion of targeted biosynthetic genes in . f. sp. ..

湿润

Investigating Fungal Biosynthetic Pathways Using , as a Heterologous Host,industries. Recently, heterologous expression has become an irreplaceable technique to functionalize fungal biosynthetic gene clusters and synthesize fungal natural products in various chassis organisms. This chapter describes the general method of using . as a chassis host to investigate fungal biosynthetic pathways.

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