Titlebook: Engineering Natural Product Biosynthesis; Methods and Protocol Elizabeth Skellam Book 2022 The Editor(s) (if applicable) and The Author(s),

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Fermi surface of hexagonal tungsten carbidesmid, effectively replacing the wild-type gene with the disrupted gene from the plasmid. The . cassette combined with the . gene serve as positive selection to identify double crossover mutants (Cai and Wolk (1990), 172(6):3138–3145, J. Bacteriol). Finally, the functional genes are cloned into anoth

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https://doi.org/10.1007/978-1-4684-8947-7 Here, we describe the process of PCR-amplifying fungal gene clusters and re-assembling them in a cloning vector via target-associated recombination in .. The gene cluster-carrying construct is validated and used to transform protoplasts of ., a well-studied host that is able to express the gene clu

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Early Evolution of the Atmosphere and Ocean, are often found in fungal species that are genetically intractable or difficult to cultivate, heterologous expression is increasingly being used for compound discovery. In addition, heterologous expression is a useful strategy to elucidate the function of the genes within a BGC and shed light on th

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