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Titlebook: Engineering Natural Product Biosynthesis; Methods and Protocol Elizabeth Skellam Book 2022 The Editor(s) (if applicable) and The Author(s),

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书目名称Engineering Natural Product Biosynthesis
副标题Methods and Protocol
编辑Elizabeth Skellam
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Engineering Natural Product Biosynthesis; Methods and Protocol Elizabeth Skellam Book 2022 The Editor(s) (if applicable) and The Author(s),
描述.This volume highlights natural products, molecular methods for identifying, and current trends in designing non-natural natural products. Chapters guide readers through protocols on heterologous expression techniques, gene disruption, modified pathway regulators, and in-vitro studies. Written in the format of the highly successful .Methods in Molecular Biology .series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols...Authoritative and cutting-edge, Engineering Natural Product Biosynthesis: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge. .Chapter 13 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com..
出版日期Book 2022
关键词Bioactive metabolites; Drug discovery; Transcriptomes; Bioengineering; Non-natural natural products
版次1
doihttps://doi.org/10.1007/978-1-0716-2273-5
isbn_softcover978-1-0716-2275-9
isbn_ebook978-1-0716-2273-5Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
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Cyanobacterial Genome Sequencing, Annotation, and Bioinformatics,the chapter, we briefly introduce genome mining tools and one successful genome mining example from our laboratory. This chapter provides general guidance regarding the sequencing project and thus includes several references for alternative methods and tools so that the reader can easily modify the
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Double Crossover Approach to Inactivate Target Gene in Cyanobacteria,smid, effectively replacing the wild-type gene with the disrupted gene from the plasmid. The . cassette combined with the . gene serve as positive selection to identify double crossover mutants (Cai and Wolk (1990), 172(6):3138–3145, J. Bacteriol). Finally, the functional genes are cloned into anoth
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