Titlebook: Electron Tomography; Methods for Three-Di Joachim Frank Book 2006Latest edition Springer-Verlag New York 2006 biology.cell.cell biology.cry

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Fiducial Marker and Hybrid Alignment Methods for Single- and Double-axis Tomography,gned so that each represents a projection of the same 3D object at a known projection angle. Inadequate image alignment will result in blurring or smearing of features in the reconstruction. The problem is made more difficult because exposure to the electron beam during the acquisition of a tilt ser

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Markerless Alignment in Electron Tomography,hough most are automated devices today, the imaging systems have certain non-idealities which give rise to abrupt shifts, rotations and magnification changes in the images. Thus, the geometric relationships between the object and the obtained projections are not precisely known initially. In this ch