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Titlebook: Electron Tomography; Methods for Three-Di Joachim Frank Book 2006Latest edition Springer-Verlag New York 2006 biology.cell.cell biology.cry

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发表于 2025-3-21 16:37:14 | 显示全部楼层 |阅读模式
书目名称Electron Tomography
副标题Methods for Three-Di
编辑Joachim Frank
视频video
概述Presents the mathematical background and working methods for three-dimensional reconstruction from projections.Takes the reader from biological specimen preparation to three-dimensional images of the
图书封面Titlebook: Electron Tomography; Methods for Three-Di Joachim Frank Book 2006Latest edition Springer-Verlag New York 2006 biology.cell.cell biology.cry
描述.Electron tomography has become a standard technique with applications in cell biology, structural biology, and materials science. This definitive work provides a comprehensive treatment of the mathematical background and working methods of three-dimensional reconstruction from tilt series, with special emphasis on the problems presented by limitations of data collection in the transmission electron microscope. In addition to chapters that are applicable to 3D reconstruction in all fields of science, such as radiological imaging in medicine and electron tomography in materials science, Electron Tomography also focuses on specimen preparation and imaging unique to biological electron microscopy. ..This extensively revised second edition updates key contributions on the mathematics of 3D reconstruction, and includes new topics such as automated tomography, frozen sectioning of cells, and the interpretation of density maps through methods of fitting, docking, denoising, and segmentation. Each chapter is a self-contained treatise by a world expert in the author‘s field of research, resulting in an indispensable resource and companion for laboratories that practice electron tomography o
出版日期Book 2006Latest edition
关键词biology; cell; cell biology; crystal; electron microscopy; materials science; microscopy; structural biolog
版次2
doihttps://doi.org/10.1007/978-0-387-69008-7
isbn_softcover978-1-4419-2172-7
isbn_ebook978-0-387-69008-7
copyrightSpringer-Verlag New York 2006
The information of publication is updating

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Segmentation of Cell Components Using Prior Knowledge,llular components .. When specimen preparation is optimized by use of rapid freezing, and imaged either directly in the frozen-hydrated state, or after freeze substitution and plastic embedding, electron tomography provides a relatively high-resolution view of biological structure in a native, or near-native, cellular context.
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How Press Propaganda Paved the Way to Brexitapter, . refers to the computation of the projection geometry of the tilt series so that most of the above deviations from the assumed ideal projection geometry could be rectified by using simple 2D geometric transformations for the images before computing a tomographic reconstruction.
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Time Runs Out on the Wholesome Warrior, their cellular environment. The resolution that can be obtained with cryotomography depends on several fundamental and technical issues related to specimen preparation, microscopy and subsequent image processing steps, but will typically be in the range of 5–10 nm.
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Cryotomography: Low-dose Automated Tomography of Frozen-hydrated Specimens, their cellular environment. The resolution that can be obtained with cryotomography depends on several fundamental and technical issues related to specimen preparation, microscopy and subsequent image processing steps, but will typically be in the range of 5–10 nm.
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