Titlebook: Electron Tomography; Methods for Three-Di Joachim Frank Book 2006Latest edition Springer-Verlag New York 2006 biology.cell.cell biology.cry

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Resolution in Electron Tomography,the electron microscope). In single-particle reconstruction, a satisfactory level of the SSNR in the 3D reconstruction is achieved by including a large number of 2D projections (tens to hundreds of thousands) that are averaged during the reconstruction process. Except for rare cases (.), the angular

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Localization and Classification of Repetitive Structures in Electron Tomograms of Paracrystalline A we define a paracrystalline specimen as one with partial ordering such that one component of the specimen may be highly regular while another may be irregular due to either low occupancy, lattice irregularity or both.

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Sample Shrinkage and Radiation Damage of Plastic Sections,oscopy. The success of embedding samples in plastic lies in the astounding resilience of the sections in the electron microscope, albeit after initial changes. The electron microscope image results from projection of the sample density in the direction of the beam, i.e. through the depth of the sect

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Electron Tomography of Frozen-hydrated Sections of Cells and Tissues,imaging method provides full 3D structural information at much higher resolution (typically 5–10 nm) than is attainable by light microscopy, and can be applied to cells and organelles that are maintained in a state that is as close to native as can be achieved currently in electron microscopy. Not o

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The Electron Microscope as a Structure Projector,cial is the belief that what is detected is some kind of projection through the structure. This ‘projection’ need not necessarily be a (weighted) sum or integral through the structure of some physical property of the latter; in principle, a monotonically varying function would be acceptable, althoug