Titlebook: Diagnosis of Sexually Transmitted Diseases; Methods and Protocol Colin R. MacKenzie,Birgit Henrich Book 2012 Springer Science+Business Medi

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Nabamita Nath,Dipayan Choudhuriotinylated DNA, indirect capturing to streptavidin-coated beads, and subsequent quantification by one-step non-nested qRT-PCR. Further, we provide some general guidelines on extraction and quantification of HIV and HCV in small volume whole blood samples.

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Guidelines for the Qualitative Detection of Viral Genomes in Dried Blood Spots,ies. DBS blood collection can be employed in the diagnosis of viral infections by PCR or RT-PCR and also in viral genome sequencing. In addition, the advent of multiplex PCR approaches has led to further diagnostic and methodological improvements allowing simultaneous detection of two or more differ

synovium

Guidelines for the Quantification of HIV and HCV in Small Volume Whole Blood Samples,n in plasma samples. The application of small samples of capillary blood allows for application in point-of-care diagnostic testing methods..Here we describe two protocols of extracting viral RNA from small samples of whole blood by hybridization to biotinylated LNA-modified 2′-.-Methyl-RNA or to bi

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Guidelines for High-Resolution Genotyping of , Using Multilocus Sequence Analysis,tant to have adequate genotyping tools for investigating the spread of . among the population. Here, we describe a high-resolution multilocus sequence typing (MLST) system able to differentiate closely related clinical strains, which makes it ideal for short-term epidemiology and outbreak investigat

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The Molecular Diagnosis of Sexually Transmitted Genital Ulcer Disease,. Real-time PCR has further refined the technology of nucleic acid amplification with detection in a closed system and enabled multiplexing to simultaneously detect multiple pathogens. It is a versatile, fast, and high-throughput system for pathogen detection that has reduced the risk of PCR contami

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