书目名称 | Diagnosis of Sexually Transmitted Diseases |
副标题 | Methods and Protocol |
编辑 | Colin R. MacKenzie,Birgit Henrich |
视频video | |
概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia |
丛书名称 | Methods in Molecular Biology |
图书封面 |  |
描述 | .Sexually transmitted infections (STI) continue to be a major cause of morbidity and mortality, both in developed industrial countries as well as in the developing world. Human immunodeficiency virus infections and the ensuing opportunistic infections are a major drain on the human and financial resources of many countries in the developing world and even with the availability of effective treatment the epidemic is not yet contained. Screening in developing worlds is difficult and there is a need for simple reliable cheap diagnostic methods that can be performed at the point of care, on the same day and by staff with limited training. Molecular biological methods are very attractive for the diagnosis of STI since a well defined range of pathogens is responsible for the infection. .Diagnosis of Sexually Transmitted Diseases: Methods and Protocols. strives to cover the full range of molecular testing for STI. Chapters cover a variety of topics such as aspects of DNA extraction from small volume samples or difficult tissues, simple, nested or multiplex PCR, use of duplex primers or other modifications of primers and PCR conditions, sequence analysis for genotyping, denaturing gel anal |
出版日期 | Book 2012 |
关键词 | PCR methods; immunodeficiency virus infections; molecular biological methods; sexually transmitted dise |
版次 | 1 |
doi | https://doi.org/10.1007/978-1-61779-937-2 |
isbn_softcover | 978-1-4939-5885-6 |
isbn_ebook | 978-1-61779-937-2Series ISSN 1064-3745 Series E-ISSN 1940-6029 |
issn_series | 1064-3745 |
copyright | Springer Science+Business Media New York 2012 |