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Titlebook: Diagnosis of Sexually Transmitted Diseases; Methods and Protocol Colin R. MacKenzie,Birgit Henrich Book 2012 Springer Science+Business Medi

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书目名称Diagnosis of Sexually Transmitted Diseases
副标题Methods and Protocol
编辑Colin R. MacKenzie,Birgit Henrich
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia
丛书名称Methods in Molecular Biology
图书封面Titlebook: Diagnosis of Sexually Transmitted Diseases; Methods and Protocol Colin R. MacKenzie,Birgit Henrich Book 2012 Springer Science+Business Medi
描述.Sexually transmitted infections (STI) continue to be a major cause of morbidity and mortality, both in developed industrial countries as well as in the developing world. Human immunodeficiency virus infections and the ensuing opportunistic infections are a major drain on the human and financial resources of many countries in the developing world and even with the availability of effective treatment the epidemic is not yet contained. Screening in developing worlds is difficult and there is a need for simple reliable cheap diagnostic methods that can be performed at the point of care, on the same day and by staff with limited training. Molecular biological methods are very attractive for the diagnosis of STI since a well defined range of pathogens is responsible for the infection. .Diagnosis of Sexually Transmitted Diseases: Methods and Protocols. strives to cover the full range of molecular testing for STI. Chapters cover a variety of topics such as aspects of DNA extraction from small volume samples or difficult tissues, simple, nested or multiplex PCR, use of duplex primers or other modifications of primers and PCR conditions, sequence analysis for genotyping, denaturing gel anal
出版日期Book 2012
关键词PCR methods; immunodeficiency virus infections; molecular biological methods; sexually transmitted dise
版次1
doihttps://doi.org/10.1007/978-1-61779-937-2
isbn_softcover978-1-4939-5885-6
isbn_ebook978-1-61779-937-2Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2012
The information of publication is updating

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William Whitehead,J. Chase McNeileproducibility, and reduced contamination. Here we describe a cost-effective and highly sensitive HBV real-time quantitative assay based on the light upon extension real-time PCR platform and a simple and reliable HBV DNA preparation method using silica-coated magnetic beads.
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Methods in Molecular Biologyhttp://image.papertrans.cn/d/image/270631.jpg
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978-1-4939-5885-6Springer Science+Business Media New York 2012
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Protocol for the Use of Enzyme-Linked Hybridization Assays for Genital Ulcer Disease,ction of microbial pathogens. In this protocol, we describe the simultaneous detection of these five pathogens and an internal control using a single-tube multiplex PCR and colorimetric enzyme-linked amplicon hybridization assay.
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