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Titlebook: Diagnosis of Sexually Transmitted Diseases; Methods and Protocol Colin R. MacKenzie,Birgit Henrich Book 2012 Springer Science+Business Medi

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Protocol for the Use of Light Upon Extension Real-Time PCR for the Determination of Viral Load in Heproducibility, and reduced contamination. Here we describe a cost-effective and highly sensitive HBV real-time quantitative assay based on the light upon extension real-time PCR platform and a simple and reliable HBV DNA preparation method using silica-coated magnetic beads.
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Nabamita Nath,Dipayan Choudhurin in plasma samples. The application of small samples of capillary blood allows for application in point-of-care diagnostic testing methods..Here we describe two protocols of extracting viral RNA from small samples of whole blood by hybridization to biotinylated LNA-modified 2′-.-Methyl-RNA or to bi
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https://doi.org/10.1007/978-3-030-26837-4. Real-time PCR has further refined the technology of nucleic acid amplification with detection in a closed system and enabled multiplexing to simultaneously detect multiple pathogens. It is a versatile, fast, and high-throughput system for pathogen detection that has reduced the risk of PCR contami
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Healthcare Technology Innovation Adoptions cervicitis and there is increasing evidence that it is causing pelvic inflammatory disease (PID). Nucleic acid amplification tests are currently the only available methods for detection, but no commercially available tests have been thoroughly evaluated. Here we describe a TaqMan-based PCR test fo
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