Titlebook: Detection of Cell Death Mechanisms; Methods and Protocol Ayesha B. Alvero,Gil G. Mor Book 2021 Springer Science+Business Media, LLC, part o

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A Triple-Parameter-Based Laboratory-Friendly Fluorescence Imaging to Identify Apoptosis in Live Celers of apoptosis. Here, we present a detailed methodology for a triple-parameter-based co-fluorescence imaging to identify apoptosis in live tumor cells. The procedure involves co-fluorescence staining specific for three cardinal features of apoptosis in live cells. The procedure is simple, time-sen

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Flow Cytometric Analyses of p53-Mediated Cell Cycle Arrest and Apoptosis in Cancer Cells, with DNA damaging agents. The method can be easily adapted to other genes of interest to study their contributions to the fate of variety of cell types in response to endogenous or exogenous stresses.

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1064-3745 ding known pitfalls... Authoritative and accessible, .Detection of Cell Death Mechanisms: Methods and Protocols .aims to ensure seamless execution of protocols on specific cell death type..978-1-0716-1164-7978-1-0716-1162-3Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Die biologischen Phasen im Leben des Weibes,tern blot analysis of caspase cleavage during apoptosis. We detail protocols for protein extraction, quantitation, casting, and running gel electrophoresis and western blot analysis of caspase -8 and caspase -9 activation. The described methods can be applied to any particular protein of interest.

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https://doi.org/10.1007/978-3-642-47656-3ers of apoptosis. Here, we present a detailed methodology for a triple-parameter-based co-fluorescence imaging to identify apoptosis in live tumor cells. The procedure involves co-fluorescence staining specific for three cardinal features of apoptosis in live cells. The procedure is simple, time-sen

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https://doi.org/10.1007/978-3-642-47656-3 with DNA damaging agents. The method can be easily adapted to other genes of interest to study their contributions to the fate of variety of cell types in response to endogenous or exogenous stresses.