Titlebook: Detection of Cell Death Mechanisms; Methods and Protocol Ayesha B. Alvero,Gil G. Mor Book 2021 Springer Science+Business Media, LLC, part o

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Subcellular Fractionation to Demonstrate Activation of Intrinsic Apoptotic Pathway,nslational modifications or loss of organelle membrane integrity lead to the movement of proteins from one compartment to another. This movement of proteins or protein translocation, exerts a significant effect on protein function. This is clearly demonstrated in the context of apoptosis wherein the

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Flow Cytometric Analyses of p53-Mediated Cell Cycle Arrest and Apoptosis in Cancer Cells,survival, such as disturbance of cell cycle progression, senescence, autophagy, and programmed cell death. One of the best examples is tumor suppressor p53. p53 functions as a tumor suppressor by inducing cell cycle arrest and apoptosis in response to genotoxic and environmental insults. The choice

mechanism

A Real-Time, Bioluminescent Apoptosis Assay,en employing traditional apoptosis detection chemistries after exposures with inducers of unknown potential. The assay continuously reports the translocation of phosphatidylserine (PS) from the inner membrane leaflet of a cell to the exofacial surface during apoptosis. This homogenous, no-wash, plat

mechanism

Detection of Anoikis Using Cell Viability Dye and Quantitation of Caspase Activity,ivation of caspases. Specific interaction between cellular receptors such as integrins and the ECM is important to maintain cellular homeostasis in normal tissues through multiple cascades. This interaction provides not only physical attachment, but more importantly, vital interaction with the actin

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In Vitro Identification and Isolation of Human Neutrophil Extracellular Traps,tuted of a DNA scaffold with associated antimicrobial proteins, which are released to the extracellular space as an effective mechanism to fight against invading microorganisms. In parallel with this beneficial role to avoid microbial dissemination and wall off infections, accumulating evidence supp