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Titlebook: Detection of Cell Death Mechanisms; Methods and Protocol Ayesha B. Alvero,Gil G. Mor Book 2021 Springer Science+Business Media, LLC, part o

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Befruchtung und Entwicklung des Eies,se transcription factors are CHOP and the spliced variant of XBP-1 (XBP1s). In this chapter, we describe a quantitative PCR method to detect the upregulation of CHOP and XBP1s mRNA during Tunicamycin-induced UPR.
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Gebärde, Laut und graphisches Zeichens were collected at different timepoints, frozen, stored, and analyzed at the end of experiments using the luminescent LDH-Glo™ Assay. High assay sensitivity and low volume sampling enabled drug-induced toxicity profiling in a time- and dose-dependent manner.
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Gebührenprivileg und Beihilferechtm one system (dependent upon selection of target gene) can be overcome through use of the second system. Strategies for optimizing the knockout process and selection of finished cell lines are also presented.
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Detection of Unfolded Protein Response by Polymerase Chain Reaction,se transcription factors are CHOP and the spliced variant of XBP-1 (XBP1s). In this chapter, we describe a quantitative PCR method to detect the upregulation of CHOP and XBP1s mRNA during Tunicamycin-induced UPR.
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