Titlebook: DNA-Protein Interactions; Principles and Proto Benoît Leblanc,Tom Moss Book 2009Latest edition Humana Press 2009 Chromatin immunoprecipitat

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Filter-Binding Assays,The concentration dependence of binding yields estimates of the equilibrium dissociation constant and trivial variations allow access to kinetic and thermodynamic data. The use of this technique is illustrated here using results from our experiments with the . methionine repressor, MetJ.

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Use of a Reporter Gene Assay in Yeast for Genetic Analysis of DNA-Protein Interactions,henotype, such as reductions or increases in the stability of the DNA–protein complex. Following identification of the relevant mutations, the mutant protein or binding site can be subjected to further analyses to confirm the expected biochemical basis of the selected phenotype. This approach has be

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Samuel Romine,Joshua Jensen,Robert BallThe concentration dependence of binding yields estimates of the equilibrium dissociation constant and trivial variations allow access to kinetic and thermodynamic data. The use of this technique is illustrated here using results from our experiments with the . methionine repressor, MetJ.

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R. Shoureshi,M. Wheeler,L. Brackneyhenotype, such as reductions or increases in the stability of the DNA–protein complex. Following identification of the relevant mutations, the mutant protein or binding site can be subjected to further analyses to confirm the expected biochemical basis of the selected phenotype. This approach has be

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Book 2009Latest editionry protocols, and notes on troubleshooting and avoiding known pitfalls....Comprehensive and authoritative, .DNA-Protein Interactions: Principles and Protocols, Third Edition. serves as an ideal guide for all those exploring this dynamic, essential, and increasingly affordable area of research..