Titlebook: DNA-Protein Interactions; Principles and Proto Benoît Leblanc,Tom Moss Book 2009Latest edition Humana Press 2009 Chromatin immunoprecipitat

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https://doi.org/10.1007/978-1-60327-015-1Chromatin immunoprecipitation; DNA; Genome-wide techniques; In vivo techniques; Microarray; PCR; Photocros

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Samuel Romine,Joshua Jensen,Robert Ball the electrophoretic mobility conferred to a DNA fragment by an interacting protein. The technique is suitable to qualitative, quantitative, and kinetic analyses. It can also be used to analyze conformational changes.

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Karina Macakaite,Arjab Singh Khumanand detected by their ability to bind radiolabeled DNA. The protein(s) interacting with the labeled DNA is visualized by autoradiography. This technique was used in our laboratory to visualize the metal regulatory consensus sequence-binding protein MTF-1 in L cell crude nuclear extracts.

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Book 2009Latest editiongenic crops, the difference between survival and starvation. In .DNA-Protein Interactions: Principles and Protocols, Third Edition., this vital subject is brought up to date with protocols exploring the most cutting-edge developments in the field, including .in vivo. and genome-wide interaction tech

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Emil Bergström,Pontus Wärneståled in the presence of the protein will feature blank regions (indicating protection) and/or enhanced cleavage sites (indicating increased availability). This technique can furthermore reveal if multiple sites for a DNA-binding protein are present on a same fragment, and allow the comparison of their respective affinities.