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Titlebook: DNA Vaccines; W. Mark Saltzman,Hong Shen,Janet L. Brandsma Book 2006 Humana Press 2006 Antigen.T cell.autoimmunity.cell.molecular biology.

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发表于 2025-3-21 16:34:18 | 显示全部楼层 |阅读模式
书目名称DNA Vaccines
编辑W. Mark Saltzman,Hong Shen,Janet L. Brandsma
视频video
丛书名称Methods in Molecular Medicine
图书封面Titlebook: DNA Vaccines;  W. Mark Saltzman,Hong Shen,Janet L. Brandsma Book 2006 Humana Press 2006 Antigen.T cell.autoimmunity.cell.molecular biology.
描述In the early 1990s, almost 200 yr after Edward Jenner demonstrated the effectiveness of the smallpox vaccine, a new paradigm for vaccination emerged. The conventional method of vaccination required delivery of whole pathogens or structural subunits, but in this new approach, DNA or genetic information was administered to elicit an immunological response. Once it was observed that plasmid DNA delivered in vivo led to production of an encoded transgene (1), two ground-breaking studies demonstrated that immunological responses could be generated against antigenic transgenes via plasmid DNA delivered by DNA vaccination (as this approach is called) (2,3). The appe- ance of this new vaccination strategy coincided with advances in molecular biology, which provided new tools to study and manipulate the basic elements of an organism’s genome and also could also be applied to the design and production of DNA vaccines. DNA Vaccines is a major updated and enhancement of the first edition. It reviews state-of-the-art methods in DNA vaccine technology, with chapters describing DNA vaccine design, delivery systems, adjuvants, current appli- tions, methods of production, and quality control. Consi
出版日期Book 2006
关键词Antigen; T cell; autoimmunity; cell; molecular biology; research; vaccination; vaccine
版次1
doihttps://doi.org/10.1385/1597451681
isbn_softcover978-1-61737-596-5
isbn_ebook978-1-59745-168-0Series ISSN 1543-1894 Series E-ISSN 1940-6037
issn_series 1543-1894
copyrightHumana Press 2006
The information of publication is updating

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Vaccination With Messenger RNA genome, no induction of autoantibodies. Moreover, mRNA which are generated by in vitro transcription, are easy to produce in large amounts and very high purity. This feature facilitates the good manufacturing practices process and guaranties batch-to-batch reproducibility. Vaccination can be achiev
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A Stress Protein-Facilitated Antigen Expression System for Plasmid DNA Vaccines-encoding sequences cloned under heterologous promoter control are delivered by techniques that lead in vivo to antigen expression in transfected cells. DNA vaccination efficiently primes both humoral and cellular immune responses. We developed a novel expression system for DNA vaccines in which a f
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In Vitro Assay of Immunostimulatory Activities of Plasmid Vectorsne is a plasmid DNA vector that can be taken up by cells to produce a protein, encoded by the vector, to be targeted for the induction of humoral or cellular responses. Although the intracellular production of the antigen may promote responses, the vectors themselves may display adjuvant activity be
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Needle-Free Delivery of Veterinary DNA Vaccinesions of DNA and multiple doses are required before an effective immune response is detected. To overcome these impediments we have developed approaches to deliver the plasmids via needle-free methods, which have been shown to be more effective than traditional needle and syringe methods. Furthermore
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A Dendrimer-Like DNA-Based Vector for DNA Delivery: A Viral and Nonviral Hybrid Approach DNA molecules are true polymers. Surprisingly, DNA molecules have not been used as a delivery vector material. This is probably due to the fact that almost all DNA have only two shapes: linear or circular. This chapter details our efforts in fabricating highly branched dendrimer-like DNA (DL-DNA) t
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