Titlebook: DNA Vaccines; Methods and Protocol Monica Rinaldi,Daniela Fioretti,Sandra Iurescia Book 2014Latest edition Springer Science+Business Media

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https://doi.org/10.1007/978-3-319-19369-4to human “Good Manufacturing Practice” (GMP) grade is mandatory, for GMP production of, e.g., viral vectors (AAV, etc.) the plasmid DNA used needs not necessarily be produced under GMP..Besides such regulatory aspects up-scaling of the plasmid DNA production process from research laboratory scale (u

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Piotr Płoński,Krzysztof Zarembamunotherapy, which relies on the injection of alum-adsorbed protein extracts, DNA vaccines do not suffer from side effects such as anaphylaxis or therapy-induced IgE antibodies. In animal models, DNA vaccines have been demonstrated to prevent TH2 sensitization or balance an existing TH2-mediated all

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https://doi.org/10.1007/978-3-319-07173-2DNA delivery to for example skin and muscle can easily be performed with electroporation. The method is efficient, feasible, and inexpensive and the future possibilities are numerous. Here we present our protocol for gene transfection to mouse skin using naked plasmid DNA and electric pulses.

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Monica Rinaldi,Daniela Fioretti,Sandra IuresciaIncludes cutting-edge methods and protocols involving DNA vaccine applications.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the exp