Titlebook: DNA Vaccines; Methods and Protocol Monica Rinaldi,Daniela Fioretti,Sandra Iurescia Book 2014Latest edition Springer Science+Business Media

Nutraceutical

书目名称DNA Vaccines影响因子(影响力)




书目名称DNA Vaccines影响因子(影响力)学科排名




书目名称DNA Vaccines网络公开度




书目名称DNA Vaccines网络公开度学科排名




书目名称DNA Vaccines被引频次




书目名称DNA Vaccines被引频次学科排名




书目名称DNA Vaccines年度引用




书目名称DNA Vaccines年度引用学科排名




书目名称DNA Vaccines读者反馈




书目名称DNA Vaccines读者反馈学科排名

Countermand

Strategies for Improving DNA Vaccine Performancestructure and easy manipulation, a wide range of strategies can be applied to improve DNA vaccine performance. This chapter discusses this topic in detail taking into account antigen/epitope selection and optimization, inclusion of intracellular targeting sequences and genetic adjuvants, and provision of T cell help.

gene-therapy

Ali Movaghar,Mansour Jamzad,Hossein Asadiep-by-step strategy for the construction of a plasmid vector with enhanced efficiency of nuclear plasmid uptake and the methodic for the in vivo efficiency evaluation of the obtained expression vector.

syncope

Rafał Grycuk,Kelton Costa,Rafał Scherer we describe fabrication methods of microneedles and DNA vaccine loading methods on the microneedle surface. Furthermore, in the latter part of this chapter, in vivo test protocols for assessing the efficacy of gene delivery using microneedles are described.

addition

Enhancement of Plasmid-Mediated Transgene Expressionep-by-step strategy for the construction of a plasmid vector with enhanced efficiency of nuclear plasmid uptake and the methodic for the in vivo efficiency evaluation of the obtained expression vector.

杀人

杀人

1064-3745 ts.Contains key notes and implementation advice from the exp.DNA Vaccines: Methods and Protocols, Third Edition .explores innovative approaches and technologies used to design, deliver, and enhance the efficacy of DNA vaccines.  Featuring applications which should be of great value in moving vaccine

弄污

机构

Mostafa Borhani,Hassan Ghassemianaccines was tested firstly in vitro, in Chinese hamster ovary cells, through flow cytometry and real-time polymerase chain reaction analysis. Then, an essential in vivo study was performed in chickens, in order to evaluate the efficacy of DNA prototype vaccines, by measuring the antibody production by enzyme-linked immunosorbent assay.

惩罚

https://doi.org/10.1007/978-981-97-0341-8how systemic and mucosal samples are prepared for analysis, followed by (4) two immunological assays: multicolor intracellular cytokine staining and tetramer/homing maker analysis that are used to evaluate effective systemic and mucosal T cell immunity.