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Titlebook: DNA Vaccines; Methods and Protocol Monica Rinaldi,Daniela Fioretti,Sandra Iurescia Book 2014Latest edition Springer Science+Business Media

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https://doi.org/10.1007/978-3-319-10849-0structure and easy manipulation, a wide range of strategies can be applied to improve DNA vaccine performance. This chapter discusses this topic in detail taking into account antigen/epitope selection and optimization, inclusion of intracellular targeting sequences and genetic adjuvants, and provision of T cell help.
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DNA Vaccines978-1-4939-0410-5Series ISSN 1064-3745 Series E-ISSN 1940-6029
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Reza Hallaj,Reza Mohseni,Kamran Kazemio make up for this limitation by altering plasmid construction through vector design innovations intended for enhancement of transgene expression and immunogenicity. The next-generation vectors also address safety issues such as selection markers. This chapter discusses (a) plasmid backbone design,
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Kusum Pandey,N. L. Kushwaha,K. G. Singhr presents methodologies for the design and cloning of synthetic antigen genes into RNA-OUT encoding antibiotic-free DNA vaccine vectors that are additionally optimized to improve protein expression, and immunogenicity, compared to alternative kanamycin-resistant vectors..First, antigen targeting co
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Marek Palkowski,Wlodzimierz Bieleckige mammals and humans, an enhancing delivery technology is required. Electroporation is a physical technique which results in improved delivery of large molecules through the cell membrane. In the case of plasmid DNA, electroporation enhances both the uptake and expression of the delivered DNA. The
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