Titlebook: DNA Sequencing Protocols; Hugh G. Griffin,Annette M. Griffin Book 19931st edition Springer Science+Business Media New York 1993

nonsensical

Hippocampus

pacifist

https://doi.org/10.1007/978-3-031-08333-4ert for digestion and to protect the primer site. The “Cyclone Sequencing” technique of Dale, McClare, and Houchins (.) overcomes this problem as it is totally independent of the restriction sites in the insert. In this procedure single-stranded Ml3 is hybridized to an oligomer that spans the .dIII

使苦恼

多骨

K. Bennaceur,Z. Sahraoui,M. A. Nacerherefore, it became the method of choice to obtain several hundred bases of sequence information per reaction. The procedure is based on the enzymatic elongation and radioactive labeling of oligonucleotides that are complementary to the beginning of the single-stranded DNA template. Chain extension

curriculum

训诫

Sesan Akintade,Seongtae Kim,Kaushik Royng a DNA polymerase (.). Several different enzymes are available for this purpose each having different qualities and properties. The use of the Klenow fragment of DNA polymerase I, DNA polymerase, and T7 DNA polymerase in DNA sequence analysis is described elsewhere in this volume.

WAX

看法等

figment