Titlebook: DNA Sequencing Protocols; Hugh G. Griffin,Annette M. Griffin Book 19931st edition Springer Science+Business Media New York 1993

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Sequential Deletions of Single-Stranded DNA,nd complementary to the homopolymer tail, is reannealed across the restriction site and the homopolymer tail. After ligation the DNA is used to transform competent cells and the resulting plaques picked at random for screening.

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Dideoxy Sequencing Reactions Using T7 Polymerase,the infrequent but specific termination by a ddNTP. This leads to a mixture of DNA fragments of varying lengths that all possess the same 5‘-end owing to the common primer. Since one of the dNTPs contains a radioactive isotope (either .P or .S) these fragments become radioactively labeled and can be

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Dideoxy Sequencing Reactions Using Taq Polymerase,Biochemicals, Cleveland, OH) and the . multiwell. (Amersham, Arlington Heights, IL) kits, both of which gave comparably good results. However these kits are relatively expensive and similar results can be obtained by preparing the different solutions with little loss of time. Here we describe a prot

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Increment

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Andrés Iglesias,Akemi Gálvez,Marta Collantessingle-stranded DNAmolecules that differ in length by only one nucleotide. Denaturing polyacrylamide gels have been reported to give interpretable separation of molecules up to 0.6 kb in length, on l-m long gels (.).

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Methods in Molecular Biologyhttp://image.papertrans.cn/d/image/260196.jpg

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Cloning into M13,ation method of sequencing DNA (.,.). General aspects of bacteriophage M13 as a cloning vector system are reviewed in ., and the preparation of foreign DNA fragments for M13 cloning is described in .. In this chapter the preparation of M13 vectors and the ligation of foreign DNA fragments (inserts) into M13 vectors are described.