| 书目名称 | DNA Sequencing Protocols | | 编辑 | Hugh G. Griffin,Annette M. Griffin | | 视频video | http://file.papertrans.cn/261/260196/260196.mp4 | | 丛书名称 | Methods in Molecular Biology | | 图书封面 |  | | 描述 | The purpose of DNA Sequencing Protocols is to provide detailed practical procedures for the widest range of DNA sequencing meth ods, and we believe that all the vanguard techniques now being applied in this fast-evolving field are comprehensively covered. Sequencing technology has advanced at a phenomenal rate since the original methods were first described in the late 1970s and there is now a huge variety of strategies and methods that can be employed to determine the sequence of any DNA of interest. More recently, a large number of new and innovative sequencing techniques have been developed, including the use of such novel polymerases as Tag poly merase and Sequenase, the harnessing of PCR technology for linear amplification (cycle) sequencing, and the advent of automated DNA sequencers. DNA sequencing is surely one of the most important techniques in the molecular biology laboratory. Sequence analysis is providing an increasingly useful approach to the characterization of biological systems, and major multinational projects are already underway to map and sequence the entire genome of organisms, such as Escherichia coli, Saccharomyces cerevisiae, Caenorhabditis elegans, and H | | 出版日期 | Book 19931st edition | | 版次 | 1 | | doi | https://doi.org/10.1385/0896032485 | | isbn_ebook | 978-1-59259-510-5Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Springer Science+Business Media New York 1993 |
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