Titlebook: DNA Repair Protocols; Daryl S. Henderson Book 1999 Humana Press 1999 DNA.Ligation.PCR.Quantitative PCR.cell lines.genes.saccharomyces cere

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The Difficult Primary Total Hip Arthroplastyision repair (NER). Each disease is subdivided into multiple complementation groups. Identification of the genetic group to which a patient belongs is relevant for both clinical and basic research aims: (1) to study relationships between genotype and phenotype, in order to understand the different c

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Joseph Borrelli Jr.,Jeffrey O. Anglenmutations. When mismatch repair is defective, cells exhibit elevated rates of spontaneous mutations. For example, microsatellite sequences exhibit frequent gains or losses of simple repeat units in tumor cells from patients with hereditary nonpolyposis colon cancer (HNPCC; .–.). Defects in mismatch

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John D. Adams Jr.,Brett D. Cristrs (CPDs) in total genomic DNA from any organism. The DNA does not have to be especially intact nor is it necessary to have any sequence information. Although the protocol given below is for measuring repair in budding yeast, the method has been successfully used to measure repair in fission yeast,

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Mark E. Morrey,Bernard F. Morrey damages in DNA. Understanding the biological impact of these agents requires quantitation of the levels of such damages in laboratory test systems as well as in field or clinical samples. Alkaline gel electrophoresis provides a sensitive (down to ∼2 lesions/5 Mb), rapid method of direct quantitatio

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