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Titlebook: DNA Repair Protocols; Daryl S. Henderson Book 1999 Humana Press 1999 DNA.Ligation.PCR.Quantitative PCR.cell lines.genes.saccharomyces cere

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Isolating Mutants of the Nematode , That Are Hypersensitive to DNA-Damaging Agents availability of thousands of mutants as well as the existence of a physical map whose sequencing (over 82 Mb finished at present) is scheduled for completion in 1999. Developmental studies have been advantaged by the animal’s transparent nature, facilitating complete elucidation of . largely invariant cell lineage.
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Isolating DNA Repair Mutants of ,ecular cloning (.–.), and to study cloned gene functions by transformation (.). Cytological studies of flies also have reached new levels of sophistication in keeping with recent advances in microscopy, probe technology, and electronic imaging (.,.).
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A Dot Blot Immunoassay for UV Photoproductshuman cells, archaebacteria, ., and plants (.–.; and McCready, unpublished). The assay is sufficiently sensitive to measure damage induced by 10 J/m. of UV-C with ease and could be used for lower doses.
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Measurement of UV Radiation-Induced DNA Damage Using Specific Antibodiesed even with very small quantities of antigen. If an antibody that recognizes DNA damage is available, either small or large damage levels can be measured using materials and equipment that are commonly available in molecular biology laboratories.
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Book 1999tein biochemistry, and genetics. Notable achievements include the molecular cloning of multiple genes associated with classical human repair disorders, such as xeroderma pigmentosum, Cockayne syndrome, and ataxia telangiectasia; elucidation of the core reaction of nucleotide excision repair (NER); t
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Methods in Molecular Biologyhttp://image.papertrans.cn/d/image/260181.jpg
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