Titlebook: DNA Methylation Protocols; Ken I. Mills,Bernard H. Ramsahoye Book 20021st edition Humana Press 2002

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Balaram Ghosal,Richard Sigliano,Y. KunimatsuCpG (where p denotes a phosphate group) (.). CpG sites are distributed throughout the genome with clusters of the sequence being found in the 5′ promoter region of housekeeping genes, in groups known as CpG islands. These short stretches of DNA have a have a C and G base composition, which in mammal

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https://doi.org/10.1057/978-1-137-59834-9r it is biological amplification by cloning in ., direct amplification by polymerase chain reaction (PCR), or signal amplification by hybridization with a probe that can be visualized. Since DNA methylation is added postreplicatively by a dedicated maintenance DNA methyltransferase that is not prese

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Katja Mielke,Anna-Katharina Hornidgeal conditions or in dissimilar cell or tissue types (.–.). High-throughput DNA array technologies have further advanced these analyses, providing for simultaneous examinations of expressions of thousands of genes between two different cell types in a single experiment (.–.). Recently, we have adapte

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Katja Mielke,Anna-Katharina Hornidge5′ CpG islands is associated with transcriptional inactivation of the involved gene. Aberrant methylation of CpG islands is an important mechanism of gene inactivation in cancer (.,.) and other states such as aging and age-related diseases (.). Such methylation often involves numerous CpG islands, a

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Katja Mielke,Anna-Katharina Hornidgehat are free of methylation, and these are known as CpG islands (.. and 2 for reviews). In addition to distinctive DNA characteristics, CpG islands also have an open chromatin structure in that they are hyperacetylated, lack histone H1, and have a nucleosome-free region (.. The major reason for inte

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Katja Mielke,Anna-Katharina Hornidgeylation is associated with imprinting, carcinogenesis, silencing of repetitive DNA, and allows for differentiating cells to efficiently shut off unnecessary genes. In vertebrates, where 60–90% of genomic CpG dinucleotides are methylated, methylation-dependent repression is vital for proper embryonic

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https://doi.org/10.1007/978-3-642-24996-9he reaction are nonmethylated cytosine in the dinucleotide CpG and methanol (.). The study of the proteins involved in demethylation requires an assay for measuring enzymatic DNA demethylation. A number of assays were previously described for determining the state of methylation of CpG sequences DNA

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