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Titlebook: DNA Methylation Protocols; Ken I. Mills,Bernard H. Ramsahoye Book 20021st edition Humana Press 2002

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书目名称DNA Methylation Protocols
编辑Ken I. Mills,Bernard H. Ramsahoye
视频video
概述Includes supplementary material:
丛书名称Methods in Molecular Biology
图书封面Titlebook: DNA Methylation Protocols;  Ken I. Mills,Bernard H. Ramsahoye Book 20021st edition Humana Press 2002
描述DNA Methylation Protocols offer a set of readily reproducible protocols of the analysis of DNA methylation and methylases. These powerful methods provide the tools necessary for studying methylation at both the global level and the level of sequence, and include many techniques for identifying genes that might be aberrantly methylated in cancer and aging. Additional methods cover genome-wide analysis of abnormal DNA methylationand the isolation and measurement of demethylases and related proteins.
出版日期Book 20021st edition
版次1
doihttps://doi.org/10.1385/1592591825
isbn_ebook978-1-59259-182-4Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 2002
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Purification of MeCP2-Containing Deacetylase from ,,diate transcriptional repression. MeCP1 is a relatively uncharacterized complex that requires at least 12 symmetrical methyl-CpGs for DNA binding (.). MeCP2 is a single polypeptide containing a methyl-binding domain capable of binding a single methyl-CpG, and a transcriptional repression domain (.).
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Measuring DNA Demethylase Activity In Vitro,te between DNA that is directly demethylated, and repair processes that remove methylated cytosines in DNA and replace them with other unmethylated cytosines found in the extracts. An additional assay is the bisulfite-mapping method, which can determine the state of methylation of cytosines at a sin
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Extracting DNA Demethylase Activity from Mammalian Cells,tivity and we defined the reactants and products of the demethylation reaction. The demethylation reaction involves the hydrolytic cleavage of the bond between the methyl-carbon and the carbon at the 5 position of the cytosine ring (. and .) producing unmethylated cytosine while the methyl group is
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