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Titlebook: DNA Damage Responses; Methods and Protocol Nima Mosammaparast Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive li

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Catherine Harrison,Thorsten Allers are slow, and FRET assays can suffer from interference and distance limitations. Here we describe an alternative methodology to monitor nuclease activity by measuring the small-angle X-ray scattering (SAXS) interference pattern from gold nanoparticles (Au NPs) conjugated to 5′-ends of dsDNA using X
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Diego Rivera Gelsinger,Jocelyne DiRuggierologous DNA pairing. This includes two distinct biochemical assays, namely, D-loop formation and synaptic complex assembly. These methods are invaluable for studying the BRCA1-BARD1 complex and its functional interplay with other factors in the HR process.
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