Titlebook: DNA Damage Responses; Methods and Protocol Nima Mosammaparast Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive li

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Use of High-Performance Liquid Chromatography-Mass Spectrometry (HPLC-MS) to Quantify Modified Nuclof interest and still maintaining exquisite specificity. In this chapter, we demonstrate how to use HPLC-MS to analyze the catalytic activity of a nucleic acid demethylase, to quantify the prevalence of .-methyladenosine from RNA, and to determine the kinetics of alkylation damage repair.

作茧自缚

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Harpoon

小故事

EWE

Data Acquisition and Getting Data into GIS,on chromatin immunoprecipitation (GLASS-ChIP) protocol. This method, combined with real-time PCR or next-generation sequencing, can identify sites of MRN endonucleolytic cutting adjacent to DNA-PK binding sites in human cells.

Ordeal

Assessing DNA Damage Responses Using B Lymphocyte Cultures,mental systems with diverse applications for studying DNA damage responses. This chapter describes methods for generating these cellular systems, inducing and quantifying DSBs, and assessing DNA damage programs.

OASIS

Targeted Formation of 8-Oxoguanine in Telomeres,formation of the common oxidative lesion 8-oxo-guanine. Here, we describe this tool and detail how to generate cell lines which express FAP-mCER-TRF1 at telomeres and verify the formation of 8-oxo-guanine.

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