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Titlebook: DNA Damage Responses; Methods and Protocol Nima Mosammaparast Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive li

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发表于 2025-3-21 19:31:17 | 显示全部楼层 |阅读模式
书目名称DNA Damage Responses
副标题Methods and Protocol
编辑Nima Mosammaparast
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: DNA Damage Responses; Methods and Protocol Nima Mosammaparast Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive li
描述.This volume provides detailed methods and key approaches used to .m.echanistically study DNA damage, as well as the factors involved in the damage response. Chapters guide readers through proteomics and biophysical approaches, analyzing protein function, quantifying DNA replication dynamics and nucleic acid base damage, as well as biochemical reconstitution of key pathways involved in DNA repair. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.. Authoritative and cutting-edge, .DNA Damage Responses: Methods and Protocols .aims to be a useful practical guide to researches to help further their study in this field..
出版日期Book 2022
关键词mass spectrometry; cytosine deaminases; DNA damage response; CRISPR/Cas9; chemoptogenetics
版次1
doihttps://doi.org/10.1007/978-1-0716-2063-2
isbn_softcover978-1-0716-2065-6
isbn_ebook978-1-0716-2063-2Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
The information of publication is updating

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Purification of DNA-Dependent Protein Kinase Catalytic Subunit (DNA-PKcs) from HeLa Cells,r, DNA-PKcs is relatively abundant in human cells, making it possible to purify the endogenous protein. Here we describe a method to purify DNA-PKcs and its binding partner Ku70/80 from HeLa cells and describe conditions for transfer of DNA-PKcs and other large polypeptides for immunoblotting.
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Juan Manuel Ruiz-Lozano,Ricardo Arocaficient interpretation. The ability to write and apply simple computing scripts propels the investigator beyond the boundaries of online analysis tools to more broadly interrogate laboratory experimental data and to integrate them with all available datasets to test and challenge hypotheses. Here we
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The Arc as Part of an Electric Circuits dictated in part by whether the broken DNA ends are resected to generate extended single-stranded DNA (ssDNA) overhangs, which are quickly bound by the trimeric ssDNA binding complex RPA, the first step of HR. Here we describe a series of protocols for generating Abelson murine leukemia virus-tran
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https://doi.org/10.1007/978-3-642-85652-5ubsequent identification of the proteins by proteomic analysis enables unbiased biochemical characterization of their associated partners, potentially revealing the physiological or functional context of any given protein. Here, we use immunoaffinity isolation of the Activating Signal Co-integrator
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The Arc as Part of an Electric Circuit study dynamic DNA repair proteins. As toxic and mutagenic repair intermediates need to be prevented from inadvertently harming the cell, DNA repair proteins often chaperone these intermediates through dynamic conformations, coordinated assemblies, and allosteric regulation. By measuring structural
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E. Benavent,A. Corberán,J. M. Sanchislular responses to these DNA breaks has established important insights into B cell development and, more broadly, has provided fundamental advances into the molecular mechanisms of DNA damage response pathways. Abelson transformed pre-B cell lines and primary pre-B cell cultures are malleable experi
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Richard W. Eglese,Adam N. Letchford, which is termed termination, is relatively unexplored. Our knowledge of termination is limited by cellular approaches to study DNA replication, which cannot readily detect termination. In contrast, the . egg extract system allows for all of DNA replication to be readily detected. Here we describe
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