Titlebook: cryoEM; Methods and Protocol Tamir Gonen,Brent L. Nannenga Book 2021 Springer Science+Business Media, LLC, part of Springer Nature 2021 cry

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Shane D. Ross,Phanindra Tallapragadaules adopt many different conformations, traditional image processing approaches often lead to blurred reconstructions. By considering complexes to be comprised of multiple, independently moving rigid bodies, multi-body refinement in RELION enables structure determination of highly flexible complexe

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Shane D. Ross,Phanindra Tallapragadalue for global resolution is insufficient to accurately describe the quality of a reconstructed density. We describe the estimation and evaluation of two additional resolution measures, local and directional resolution, using methods based on the Fourier shell correlation (FSC). We apply the protoco

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Santo Banerjee,Lamberto Rondonisoluble and membrane proteins, employing identical principles and methods once 2D crystals are obtained. Two-dimensional crystallization trials of membrane proteins can result in multiple outcomes of ordered arrays, which may be suited for either 2D electron crystallography, helical analysis, or Mic

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Santo Banerjee,Lamberto Rondonientional diffraction techniques. MicroED has been used to determine the structures of many proteins and small organic molecules, and the technique can be performed on most standard cryo-TEM instruments equipped with high-speed detectors capable of collecting electron diffraction data. Here, we prese

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