Titlebook: cryoEM; Methods and Protocol Tamir Gonen,Brent L. Nannenga Book 2021 Springer Science+Business Media, LLC, part of Springer Nature 2021 cry

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1064-3745 itative and cutting-edge, .CryoEM: Methods and Protocols .aims to serve as an excellent resource on cryo-EM and can serve as the foundation for new researchers to this growing field in structural biology. .978-1-0716-0968-2978-1-0716-0966-8Series ISSN 1064-3745 Series E-ISSN 1940-6029

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https://doi.org/10.1007/978-3-642-21922-1protocol will allow the reader to obtain data that can be used for morphological analysis and precise measurements of biological structures in their cellular environment. Furthermore, this data can be used for more elaborate structural analysis by applying further image processing steps like subtomo

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M. P. Hanias,H. E. Nistazakis,G. S. Tombrasspecimen was illuminated with a parallel electron beam. Here, this chapter describes how to establish parallel illumination conditions in a Talos Arctica TEM for high-resolution cryogenic data collection for structure determination.

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Santo Banerjee,Lamberto Rondonierated by submicron filter paper and mechanical means for the separation of stacked 2D crystals into single-layered 2D crystals, for which standard 2D electron crystallography can then be employed. The preparation of 2D crystals, the peel-blot grid preparation, and the structure determination by 2D

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Setting Up Parallel Illumination on the Talos Arctica for High-Resolution Data Collectionspecimen was illuminated with a parallel electron beam. Here, this chapter describes how to establish parallel illumination conditions in a Talos Arctica TEM for high-resolution cryogenic data collection for structure determination.

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Low-Dose Data Collection and Radiation Damage in MicroEDge is inflicted over the course of data acquisition. Ideally, lower doses increase the measurable volume of a single-crystal lattice by reducing the damage caused by probing electrons. However, the information that can be recovered from a diffraction image is directly related to the number of electr

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Book 2021 cryotomography, single particle analysis, and the crystal based cryo-EM methods of 2D electron crystallography, and MicroED for the study of 3D crystals. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, applica