Titlebook: cAMP Signaling; Methods and Protocol Manuela Zaccolo Book 2022Latest edition The Editor(s) (if applicable) and The Author(s), under exclusi

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David Campelo,Telmo Silva,Jorge Abreug cardiomyocyte function, as well as pathological processes, by acting in distinct subcellular microdomains and thus controlling excitation–contraction coupling. Spatio-temporal intracellular dynamics of cyclic nucleotides can be measured in living cells using fluorescence resonance energy transfer

endoscopy

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https://doi.org/10.1007/978-3-319-90170-1xicity or no autofluorescence, and compatibility to deep-tissue imaging or optogenetics. However, functional imaging of cellular signaling by bioluminescence is not so easy due to the limited availability of bright bioluminescent indicators..Here we describe a detailed strategy to detect cellular cA

Cognizance

https://doi.org/10.1007/978-3-319-90170-1nsgenic mice are used as an exciting tool for in vivo or in situ analysis of fluorescent biosensors, which are capable of directly reporting second messenger levels and biochemical processes in real time and living cells. In this chapter, we present a detailed protocol for the generation of plasmid

Anthrp

David Campelo,Telmo Silva,Jorge Abreuing of physiological and pathological processes. However, the development of sensors remains an intricate process. Using simulation techniques, we recently introduced a new architecture to measure intracellular concentrations of cAMP named CUTie, which works as a FRET tag for arbitrary targeting dom

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Michele Roncalli,Alessandro Farinelliat have been modified to transduce cAMP concentrations into electrical or fluorescent readouts that can be readily detected using patch clamp amplifiers, photomultiplier tubes, or cameras. Here, we describe two complementary approaches for the detection and measurement of cAMP signals near the plasm

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Smart Cities for the Rest of Usion of key target proteins via activation of the cAMP effector protein kinase A (PKA) is achieved via signal compartmentalization. Termination of the cAMP signal is mediated by phosphodiesterases (PDEs), a diverse group of enzymes comprising several families that localize to distinct cellular compar

Boycott

Smart Cities for the Rest of Us), a Ser/Thr protein kinase. Where and when this enzyme is activated inside the cell has profound implications on the functional impact of PKA. It is now well established that PKA signaling is focused locally into subcellular signaling “islands” or “signalosomes.” The A-Kinase Anchoring Proteins (AK

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