Titlebook: cAMP Signaling; Methods and Protocol Manuela Zaccolo Book 2022Latest edition The Editor(s) (if applicable) and The Author(s), under exclusi

ACRO

书目名称cAMP Signaling影响因子(影响力)




书目名称cAMP Signaling影响因子(影响力)学科排名




书目名称cAMP Signaling网络公开度




书目名称cAMP Signaling网络公开度学科排名




书目名称cAMP Signaling被引频次




书目名称cAMP Signaling被引频次学科排名




书目名称cAMP Signaling年度引用




书目名称cAMP Signaling年度引用学科排名




书目名称cAMP Signaling读者反馈




书目名称cAMP Signaling读者反馈学科排名

dendrites

Daniel Carvalho,Telmo Silva,Jorge Abreully performed in vitro using radio-labeled ATP. For in vivo studies, genetically encoded FRET-based sensors have become popular for monitoring PKA activity. Here, we show that it is also possible to apply such reporters in vitro. We describe how to express and purify milligram quantities of a FRET-b

字形刻痕

Painstaking

Applications and Usability of Interactive TVgenetic methods utilize microbial rhodopsins to elicit neuronal de- or hyperpolarization. However, other optogenetic tools have emerged that allow influencing neuronal function by different approaches. In this chapter we describe the use of photoactivated adenylyl cyclases (PACs) as modulators of ne

Microaneurysm

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Applications and Usability of Interactive TVith PKA-repressed reporters to either carry out high throughput screens for small molecule inhibitors of these target enzymes or to assess hit compounds and their analogs from such screens. Here, we describe two methods for testing panels of such compounds. The first uses a growth assay for which gr

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vertebrate

侵略

Carina Haro Granizo,Gonzalo Olmedoiomyocytes. However, as the differentiation process is lengthy and commercially available cells are expensive, the cell number is limited. Here we provide detailed information on how to scale down 2D cell cultures of hIPS-CMs for the purpose of cAMP FRET measurements, thereby extending the number of

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