Titlebook: cAMP Signaling; Methods and Protocol Manuela Zaccolo Book 2015 Springer Science+Business Media New York 2015 Adenosine 3′,5′-monophosphate.

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Development of Computational Models of cAMP Signaling,f cAMP computational models. We describe how features of the cAMP network can be represented and review the types of experimental data useful in modeling cAMP signaling. We also compile a list of published cAMP models that can aid in the development of novel dynamical models of cAMP signaling.

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Applications and Usability of Interactive TVine a methodology to dissect kinetics of cAMP-mediated FRET signals in single cells using automated image analysis approaches. We additionally extend these approaches to the analysis of subcellular regions. These approaches offer an unique opportunity to assess localized cAMP kinetics in an unbiased, quantitative fashion.

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https://doi.org/10.1007/978-3-031-22210-8a membrane of cells. These probes take advantage of the ability of cyclic nucleotide-gated (CNG) channels to transduce small changes in cAMP concentrations into ionic flux through channel pores that can be readily detected by measuring Ca. and/or Mn. influx or by measuring ionic currents.

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Applications and Usability of Interactive TVols provide cyclase activity capable of increasing cellular cAMP levels up to a hundredfold with either . or . kinetic characteristics. Here, we consider the functional features of different cyclases and provide operating guidelines to optimize the use of PACs in vivo.

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Marc Aguilar,Pau Pamplona,Sergi Fernándezfrom growing in medium containing the pyrimidine analog 5-fluoro orotic acid (5FOA). We describe here the steps required to construct strains for screening and to optimize conditions for successful screens.