Titlebook: cAMP Signaling; Methods and Protocol Manuela Zaccolo Book 2015 Springer Science+Business Media New York 2015 Adenosine 3′,5′-monophosphate.

被告

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FUME

声明

María José Abásolo,Raoni Kuleszasfer (FRET)-based biosensor for the second messenger 3′,5′-cyclic adenosine monophosphate. These tools and techniques should provide great potential for the analysis of second messenger dynamics in a more physiologically relevant context.

愉快吗

Applications and Usability of Interactive TVng are valuable tools in the investigation of local PKA action. This article outlines the development of PKA isoform-selective disruptor peptides, documents the optimization of cell-soluble peptide derivatives, and introduces alternative cell-based approaches that interrogate other aspects of the PKA–AKAP interface.

countenance

https://doi.org/10.1007/978-3-319-38907-3re for identifying variants of PKA regulatory subunit D/D domains that bind selectively to individual AKAPs. This procedure can be adapted for engineering specificity into other shared protein interfaces.

FORGO

Jorge Abreu,Pedro Almeida,Telmo Silvaf cAMP computational models. We describe how features of the cAMP network can be represented and review the types of experimental data useful in modeling cAMP signaling. We also compile a list of published cAMP models that can aid in the development of novel dynamical models of cAMP signaling.

MEET

Simultaneous Assessment of cAMP Signaling Events in Different Cellular Compartments Using FRET-BaseAMP and its actions in subcellular compartments of neighboring cells. As proof of principle, we compare cAMP signals and PKA activity in the cytosol of neighboring HEK cells. We propose that this flexible and powerful method can significantly improve the direct comparison of cAMP signals and their action in specific cellular domains.

chandel

Structure-Based, In Silico Approaches for the Development of Novel cAMP FRET Reporters,aw simple qualitative rules that may aid the design of novel sensors. Finally, we will illustrate how the application of these simple rules can be used to describe the mechanistic basis of cAMP sensors reported in the literature.

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