| 书目名称 | Cytokine Bioassays |
| 副标题 | Methods and Protocol |
| 编辑 | Ivana Vancurova |
| 视频video | |
| 概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia |
| 丛书名称 | Methods in Molecular Biology |
| 图书封面 |  |
| 描述 | Cytokines are pleiotropic regulatory proteins involved in essentially all biological processes and associated with a wide variety of diseases, including inflammatory disorders as well as many types of cancer and leukemia. Knowledge about the quantitative and qualitative nature of cytokine production is critical in the understanding of normal and pathological processes. The cytokine detection in biological and clinical samples faces many challenges including their low abundance, the need to distinguish between active and latent cytokine forms, and the need to measure multiple cytokines in a single assay. This volume will provide a comprehensive collection of classic and cutting-edge methodologies that are currently used to analyze and quantify cytokines and their biological activities in complex biological and clinical samples. The chapters are divided into four main categories. The first group focuses on the immunodetection of released cytokines in tissue culture supernatants, plasma, serum and whole blood samples by immunoassays. These immunoassays measure the total concentrations of released cytokines regardless of their biological activity and include ELISA, flow cytometry, ELIS |
| 出版日期 | Book 2014 |
| 关键词 | Cytokines; RT-PCR; analysis of intracellular cytokines by flow cytometry; biologically active cytokines |
| 版次 | 1 |
| doi | https://doi.org/10.1007/978-1-4939-0928-5 |
| isbn_softcover | 978-1-4939-4850-5 |
| isbn_ebook | 978-1-4939-0928-5Series ISSN 1064-3745 Series E-ISSN 1940-6029 |
| issn_series | 1064-3745 |
| copyright | Springer Science+Business Media New York 2014 |
发表于 2025-3-21 16:26:53
