Titlebook: Cytokine Bioassays; Methods and Protocol Ivana Vancurova Book 2014 Springer Science+Business Media New York 2014 Cytokines.RT-PCR.analysis

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https://doi.org/10.1007/978-1-349-21483-9ents that lead to switching the immunoglobulin isotopes from IgM to IgE. This chapter describes the methodology of class switch recombination assay for assessment of the effect of the environmental pollutants in toxicological laboratory diagnostics.

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大约冬季

Assessment of Phagocytic Activity of Cultured Macrophages Using Fluorescence Microscopy and Flow Cytacrophages, which can easily be applied to other phagocytes. Each method is based on the extent of engulfment of FITC-labeled latex minibeads by macrophages under different conditions. Phagocytic activity can be assessed either by counting individual cells using a fluorescence microscope or measuring fluorescence intensity using a flow cytometer.

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Cytokine-Induced Neutrophil Chemotaxis Assayasis, interleukin-8 (IL-8) is one of the most potent PMN chemokines known. This chapter describes an in vitro chemotaxis technique using PMNs and IL-8 which can serve the investigator as an established model from which new studies can be developed.

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