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Titlebook: Crop Breeding; Methods and Protocol Delphine Fleury,Ryan Whitford Book 2014 Springer Science+Business Media New York 2014 crop improvement.

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In vitro Culture for Doubled Haploids: Tools for Molecular Breeding,Doubled haploid (DH) techniques using immature microspores or embryos rescued in vitro following interspecific hybridization are important tools for breeding wheat and barley and for generating populations for gene mapping purposes. Here, we describe methods being used for the successful production of spring wheat and barley DHs in Australia.
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qPCR for Quantification of Transgene Expression and Determination of Transgene Copy Number,Quantitative real-time PCR (qPCR) is a mature technology that can be used to accurately quantify the number of copies of a target nucleic acid in a sample. Here, we describe a method for using this technology to determine the copy number of a transgene stably integrated into a plant’s genome and to ascertain the level of transgene expression.
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Christoph Siepermann,Jan Vockerothlar marker information collected on plants under selection in a breeding program. They reflect the breeding value of the plants and help breeders to choose the best ones for next generation. . is an R package that calculates phenotypic and molecular selection indices.
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